Anticorps Polyclonal de lapin anti-NOL3

NOL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10846-2-PBS

Synonymes

Nop30, NOP, Myp, Muscle-enriched cytoplasmic protein, CARD2

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 10846-2-AP (same clone as 10846-2-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10846-2-AP (same clone as 10846-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10846-2-AP (NOL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10846-2-AP (same clone as 10846-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10846-2-AP (NOL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NOL3 antibody (10846-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10846-2-AP (same clone as 10846-2-PBS)

  Immunofluorescent analysis of HepG2 cells, using NOL3 antibody 10846-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10846-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10846-2-PBS cible NOL3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOL3 Protéine recombinante Ag1306
• Nom complet: nucleolar protein 3 (apoptosis repressor with CARD domain)
• Masse moléculaire calculée: 24 aa, 3 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC012798
• Symbole du gène: NOL3
• Identification du gène (NCBI): 8996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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