Anticorps Polyclonal de lapin anti-NOL9

NOL9 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA, FRAP

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16083-1-AP

Synonymes

NOL9, nucleolar protein 9

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Galerie de données de validation

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16083-1-AP cible NOL9 dans les applications de WB, IF, ELISA, FRAP et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOL9 Protéine recombinante Ag9082
• Nom complet: nucleolar protein 9
• Masse moléculaire calculée: 702 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC009257
• Symbole du gène: NOL9
• Identification du gène (NCBI): 79707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.

Publications

Species	Application	Title
human	FRAP	Nucleic Acids Res; Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity.; Authors - Witold Szaflarski
human	WB,IF	Mol Biol Cell; LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.; Authors - Castle Christopher D CD
human	WB	Cancer Res Commun; USP36 SUMOylates Las1L and promotes its function in pre-rRNA ITS2 processing; Authors - Yanping Li

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