Anticorps Polyclonal de lapin anti-NONO

• Phare
• Validé par KD/KO

NONO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (2)

N° de cat : 11058-1-AP

Synonymes

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

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Galerie de données de validation

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  WB analysis using 11058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11058-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11058-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human pancreas using 11058-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11058-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11058-1-AP

  IP Result of anti-NONO (IP:11058-1-AP, 4ug; Detection:11058-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11058-1-AP (NONO antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11058-1-AP (NONO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 11058-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug NONO antibody (11058-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293, cellules MCF-7, cellules NIH/3T3, cellules PC-12, tissu de muscle squelettique humain, tissu pancréatique de souris, tissu pancréatique humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

11058-1-AP cible NONO dans les applications de WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NONO Protéine recombinante Ag1512
• Nom complet: non-POU domain containing, octamer-binding
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC010049
• Symbole du gène: NONO
• Identification du gène (NCBI): 4841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB,RIP	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou ; KD Validated
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
human	WB,IHC,IF	Sci Adv; Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer.; Authors - Yuanyuan Qin ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was decent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

NONO Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11058-1-AP)

FH

Tatyana (Verified Customer) (05-14-2023)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

NONO Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11058-1-AP)

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