Anticorps Monoclonal anti-NONO

NONO Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2A2B10

N° de cat : 66361-1-Ig

Synonymes

2A2B10, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, NonO protein, Non-POU domain-containing octamer-binding protein

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Galerie de données de validation

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  WB analysis of C2C12 using 66361-1-Ig

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66361-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 66361-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66361-1-Ig (NONO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellule C2C12, cellules ROS1728, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66361-1-Ig cible NONO dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NONO Protéine recombinante Ag17609
• Nom complet: non-POU domain containing, octamer-binding
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC010049
• Symbole du gène: NONO
• Identification du gène (NCBI): 4841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Circular RNA circTmem241 drives group III innate lymphoid cell differentiation via initiation of Elk3 transcription; Authors - Nian Liu

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