Anticorps Polyclonal de lapin anti-NOP16

NOP16 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 31969-1-AP

Synonymes

NOP16 Nucleolar Protein, Hypothetical Protein HSPC111, HSPC185, HSPC111, HBV pre-S2 trans-regulated protein 3

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Galerie de données de validation

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  WB analysis using 31969-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31969-1-AP (NOP16 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules K-562, cellules MCF-7, cellules Raji
• Résultats positifs en IF/ICC: cellules A431, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31969-1-AP cible NOP16 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOP16 Protéine recombinante Ag36714
• Nom complet: NOP16 nucleolar protein homolog (yeast)
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC040106
• Symbole du gène: NOP16
• Identification du gène (NCBI): 51491
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOP16, also known as HSPC111, is found in the intracellular protein complex. It is a ribosomal protein that is transcriptionally regulated by the oncogene c-Myc. NOP16 may be involved in the completion of ribosome synthesis, therefore, reduced NOP16 expression in cancer cells may block many biological processes. Studies indicate that NOP16 is highly expressed in more than 20 types of cancers, including colorectal, breast, and hepatocellular carcinoma, and is an important target for clinical treatment of related diseases.

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