Anticorps Monoclonal anti-NOP2

NOP2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F4F4

N° de cat : 68889-1-PBS

Synonymes

Nucleolar protein 1, NSUN1, NOP120, NOL1, EC:2.1.1.-

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Galerie de données de validation

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  WB analysis using 68889-1-Ig (same clone as 68889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68889-1-Ig (NOP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68889-1-Ig (same clone as 68889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOP2 antibody (68889-1-Ig, Clone: 1F4F4 ) at dilution of 1:561 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 68889-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68889-1-PBS cible NOP2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NOP2 Protéine recombinante Ag33985
• Nom complet: NOP2 nucleolar protein homolog (yeast)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC000656
• Symbole du gène: NOP2
• Identification du gène (NCBI): 4839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13 (PMID: 23775790).

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