Anticorps Polyclonal de lapin anti-NOP56

NOP56 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 18181-1-AP

Synonymes

NOL5A, NOP 56

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 18181-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18181-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18181-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 18181-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18181-1-AP (NOP56 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules A549, cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

18181-1-AP cible NOP56 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOP56 Protéine recombinante Ag12882
• Nom complet: NOP56 ribonucleoprotein homolog (yeast)
• Masse moléculaire calculée: 594 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC104791
• Symbole du gène: NOP56
• Identification du gène (NCBI): 10528
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Box C/D RNA-protein complexes (RNPs) guide the 2'-O-methylation of nucleotides in eukaryotic ribosomal RNAs. The box C/D and C'/D' RNP subcomplexes are each assembled with three sRNP core proteins. The Nop56 core protein mediates crucial protein-protein interactions required for both sRNP assembly and the methyltransferase reaction by bridging the L7Ae and fibrillarin core proteins [PMID:22496443]. NOP56 is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2-prime-O-methylation of pre-ribosomal RNA (rRNA) during its maturation. It is predicted to function in an early to middle step in pre-rRNA processing [PMID:12777385].

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
mouse	WB	iScience; BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.; Authors - Marlene Cervantes

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