Anticorps Polyclonal de lapin anti-NOP58

NOP58 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 14409-1-AP

Synonymes

HSPC120, NOL5, NOP5, NOP5/NOP58, NOP58, Nucleolar protein 5, Nucleolar protein 58

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Galerie de données de validation

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  WB analysis using 14409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14409-1-AP (NOP58 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14409-1-AP

  IP Result of anti-NOP58 (IP:14409-1-AP, 4ug; Detection:14409-1-AP 1:500) with K-562 cells lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

14409-1-AP cible NOP58 dans les applications de WB, RIP, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOP58 Protéine recombinante Ag5740
• Nom complet: NOP58 ribonucleoprotein homolog (yeast)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC032592
• Symbole du gène: NOP58
• Identification du gène (NCBI): 51602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The box C/D snoRNPs,is named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including: RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation [PMID:9649444,8878486]. Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins [PMID:10606270].

Publications

Species	Application	Title
mouse	WB	Elife; The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.; Authors - Yick W Fong
human	WB,CoIP	J Virol; Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes.; Authors - Beatriz Alvarado-Hernandez
	CoIP,RIP	iScience; BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.; Authors - Marlene Cervantes
human	WB	Dig Dis Sci; CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58; Authors - Zhao Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marlene (Verified Customer) (02-25-2019)

Immunoprecipitation:From mouse liver lysate4ug of antibody per sampleIncubated overnight at 4C

• Applications: Immunoprecipitation,
• Cell Tissue Type: Mouse liver tissue