Anticorps Polyclonal de lapin anti-NOP58

• Phare
• Validé par KD/KO

NOP58 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 14409-1-AP

Synonymes

HSPC120, NOL5, NOP5, NOP5/NOP58, Nucleolar protein 5

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Galerie de données de validation

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  WB analysis using 14409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14409-1-AP (NOP58 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14409-1-AP

  WB result of NOP58 antibody (14409-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOP58 transfected HeLa cells.

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  IP experiment of HeLa using 14409-1-AP

  IP result of anti-NOP58 (IP:14409-1-AP, 4ug; Detection:14409-1-AP 1:8000) with HeLa cells lysate 1400 ug.

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  IHC staining of mouse kidney using 14409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14409-1-AP (NOP58 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 2 publications below

Informations sur le produit

14409-1-AP cible NOP58 dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOP58 Protéine recombinante Ag5740
• Nom complet: NOP58 ribonucleoprotein homolog (yeast)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC032592
• Symbole du gène: NOP58
• Identification du gène (NCBI): 51602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The box C/D snoRNPs,is named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including: RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation [PMID:9649444,8878486]. Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins [PMID:10606270].

Publications

Species	Application	Title
mouse	WB	Elife; The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.; Authors - Yick W Fong
human	WB,CoIP	J Virol; Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes.; Authors - Beatriz Alvarado-Hernandez
	RIP,CoIP	iScience; BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.; Authors - Marlene Cervantes
human	WB	Dig Dis Sci; CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58; Authors - Zhao Wu
human	WB,RIP,IF	EMBO Rep; N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules; Authors - Pavel Kudrin
human	WB	medRxiv; New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops; Authors - Md Lutfur Rahman

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marlene (Verified Customer) (02-25-2019)

Immunoprecipitation:From mouse liver lysate4ug of antibody per sampleIncubated overnight at 4C

• Applications: Immunoprecipitation,
• Cell Tissue Type: Mouse liver tissue

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