Anticorps Polyclonal de lapin anti-iNOS

iNOS Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

74

N° de cat : 22226-1-AP

Synonymes

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Galerie de données de validation

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  WB analysis using 22226-1-AP

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22226-1-AP (NOS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 22226-1-AP

  1X10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (22226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7,
• Résultats positifs en cytométrie: cellules RAW 264.7 traitées au LPS

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 57 publications below
• IHC: See 9 publications below
• IF: See 19 publications below

Informations sur le produit

22226-1-AP cible iNOS dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: iNOS Protéine recombinante Ag17567
• Nom complet: nitric oxide synthase 2, inducible
• Masse moléculaire calculée: 1153 aa, 131 kDa
• Poids moléculaire observé: 110-130 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: BC130283
• Symbole du gène: NOS2
• Identification du gène (NCBI): 4843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228).

Publications

Species	Application	Title
human,mouse	WB,IHC	Mol Ther; Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy; Authors - Jia-Lu Liu
mouse	WB,IF	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
mouse	IHC	Cancer Lett; Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis; Authors - Xing Li
mouse	IF	J Neuroinflammation; Disturbance of neuron-microglia crosstalk mediated by GRP78 in Neuropsychiatric systemic lupus erythematosus mice; Authors - Jingyi Xu
mouse	WB,IF	Oxid Med Cell Longev; Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2; Authors - Jie Jin
human	WB	Oxid Med Cell Longev; Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.; Authors - Xin Li