Anticorps Recombinant de lapin anti-NOS3

NOS3 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242543E10

N° de cat : 85020-4-PBS

Synonymes

eNOS, EC-NOS, ECNOS, EC:1.14.13.39, cNOS

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Galerie de données de validation

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  WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85020-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85020-4-RR against Human NOS3 were performed. The affinity constant is 3.17 nM.

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Informations sur le produit

85020-4-PBS cible NOS3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOS3 Protéine recombinante Ag25712
• Nom complet: nitric oxide synthase 3 (endothelial cell)
• Poids moléculaire observé: 140 kDa
• Symbole du gène: eNOS
• Identification du gène (NCBI): 4846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

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