Anticorps Polyclonal de lapin anti-NOSTRIN

NOSTRIN Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 20116-1-AP

Synonymes

BM247 homolog, DaIP2, eNOS trafficking inducer, NOSTRIN

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Galerie de données de validation

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  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20116-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 20116-1-AP

  IP Result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20116-1-AP

  Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque de souris, tissu hépatique de souris, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cirrhose hépatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20116-1-AP cible NOSTRIN dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOSTRIN Protéine recombinante Ag13989
• Nom complet: nitric oxide synthase trafficker
• Masse moléculaire calculée: 506 aa, 58 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC014189
• Symbole du gène: NOSTRIN
• Identification du gène (NCBI): 115677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
rat	WB	J Inflamm Res; TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters; Authors - Jufang Wang
		Naunyn Schmiedebergs Arch Pharmacol; Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway.; Authors - Mohamed Abd-Elbaset
rat	WB	Mol Med Rep; Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death.; Authors - Xiaoyan Hu
rat	WB	Mol Med Rep; UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.; Authors - Bingju Yan
human	WB	Mol Med Rep; Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.; Authors - Bingju Yan