Anticorps Polyclonal de lapin anti-NOSTRIN

NOSTRIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 20116-1-AP

Synonymes

eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog

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Galerie de données de validation

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  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20116-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 20116-1-AP

  IP result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20116-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20116-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20116-1-AP

  Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cardiaque de souris, tissu hépatique de souris, tissu placentaire humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cirrhose hépatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20116-1-AP cible NOSTRIN dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOSTRIN Protéine recombinante Ag13989
• Nom complet: nitric oxide synthase trafficker
• Masse moléculaire calculée: 506 aa, 58 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC014189
• Symbole du gène: NOSTRIN
• Identification du gène (NCBI): 115677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.

Publications

Species	Application	Title
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
rat	WB	J Inflamm Res; TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters; Authors - Jufang Wang
		Naunyn Schmiedebergs Arch Pharmacol; Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway.; Authors - Mohamed Abd-Elbaset
rat	WB	Mol Med Rep; Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death.; Authors - Xiaoyan Hu
rat	WB	Mol Med Rep; UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.; Authors - Bingju Yan
human	WB	Mol Med Rep; Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.; Authors - Bingju Yan

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