Anticorps Polyclonal de lapin anti-NOTCH1

• Phare
• Validé par KD/KO

NOTCH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

74

N° de cat : 20687-1-AP

Synonymes

hN1, Notch 1, NOTCH1, TAN1

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Galerie de données de validation

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  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20687-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20687-1-AP

  IP result of anti-NOTCH1(IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:600) with HEK-293 cells lysate 1480 ug.

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  IP experiment of HepG2 using 20687-1-AP

  IP result of anti-NOTCH1(IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:500) with HepG2 cells lysate 1360 ug.

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  IHC staining of human breast cancer using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 20687-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20687-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH1 antibody (20687-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A2780, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris, tissu de lymphome humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 60 publications below
• IHC: See 10 publications below
• IF: See 9 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

20687-1-AP cible NOTCH1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, vison
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Notch homolog 1, translocation-associated (Drosophila)
• Masse moléculaire calculée: 273 kDa
• Poids moléculaire observé: 273-300 kDa, 120 kDa
• Numéro d’acquisition GenBank: NM_017617
• Symbole du gène: NOTCH1
• Identification du gène (NCBI): 4851
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 form (120 kDa).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
mouse	IF	Mol Psychiatry; Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression.; Authors - Tong Ni
human	IHC	EBioMedicine; Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes; Authors - Fan Wu
human	WB	Cell Rep; Transcriptional control of brain tumor stem cells by a carbohydrate binding protein; Authors - Ahmad Sharanek
human,mouse	WB,IHC	Int J Cancer; Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.; Authors - Qingmin Yuan

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