Anticorps Polyclonal de lapin anti-NOTCH2

• Phare
• Validé par KD/KO

NOTCH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 28580-1-AP

Synonymes

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

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Galerie de données de validation

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  WB analysis using 28580-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 28580-1-AP (NOTCH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 28580-1-AP

  WB result of NOTCH2 antibody (28580-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and NOTCH2 knockout HEK-293T cells.

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  IP experiment of U-87 MG using 28580-1-AP

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with U-87 MG cells lysate 1960 ug.

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  IP experiment of HEK-293 using 28580-1-AP

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human stomach using 28580-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28580-1-AP (NOTCH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 28580-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NOTCH2 antibody (28580-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules HEK-293T
• Résultats positifs en IP: cellules HEK-293, cellules MG U-87
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

28580-1-AP cible NOTCH2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOTCH2 Protéine recombinante Ag29413
• Nom complet: Notch homolog 2 (Drosophila)
• Masse moléculaire calculée: 265 kDa
• Poids moléculaire observé: 110 kDa, 265 kDa
• Numéro d’acquisition GenBank: NM_024408
• Symbole du gène: NOTCH2
• Identification du gène (NCBI): 4853
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).

Publications

Species	Application	Title
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB	Life Sci; GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma.; Authors - Wei Wang
human	WB	Ann Transl Med; SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway.; Authors - Yan Feng
human	IHC	Transl Androl Urol; Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer.; Authors - Yunhao Qing
human	WB	Front Oncol; A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer; Authors - Xianyun Xu

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