Anticorps Recombinant de lapin anti-NOTCH2

• Phare
• Validé par KD/KO

NOTCH2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241162A2

N° de cat : 84074-4-RR

Synonymes

241162A2, hN2, N2ECD, N2ICD, Notch 2

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Galerie de données de validation

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  WB analysis using 84074-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84074-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84074-4-RR

  WB result of NOTCH2 antibody (84074-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOTCH2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 84074-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules A431, cellules HEK-293, cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84074-4-RR cible NOTCH2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOTCH2 Protéine recombinante Ag29413
• Nom complet: Notch homolog 2 (Drosophila)
• Masse moléculaire calculée: 265 kDa
• Poids moléculaire observé: 110 kDa, 265kDa
• Numéro d’acquisition GenBank: NM_024408
• Symbole du gène: NOTCH2
• Identification du gène (NCBI): 4853
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).

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