Anticorps Monoclonal anti-NOTCH2/NOTCH2NL

NOTCH2/NOTCH2NL Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D5E1

N° de cat : 68212-1-PBS

Synonymes

NOTCH2NL, NOTCH2NLC, NOTCH2, Notch homolog 2 N-terminal-like protein C, N2N

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Galerie de données de validation

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  WB analysis of hTERT-RPE1 using 68212-1-Ig (same clone as 68212-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 68212-1-Ig (same clone as 68212-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 68212-1-Ig (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68212-1-Ig (same clone as 68212-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68212-1-Ig (same clone as 68212-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH2/NOTCH2NL antibody (68212-1-Ig, Clone: 2D5E1 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68212-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68212-1-PBS cible NOTCH2/NOTCH2NL dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NOTCH2/NOTCH2NL Protéine recombinante Ag1679
• Nom complet: Notch homolog 2 (Drosophila) N-terminal like
• Masse moléculaire calculée: 265 kDa
• Poids moléculaire observé: 250-265 kDa
• Numéro d’acquisition GenBank: BC019835
• Symbole du gène: NOTCH2NL
• Identification du gène (NCBI): 388677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Notch 2 is a member of the notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch2, like other Norch genes, functions in development of various tissues. This antibody can recognize NOTCH2 and NOTCH2NL. The antibody detects the full-length NOTCH2 protein about 250-265 kDa.

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