Anticorps Recombinant de lapin anti-NOX1

NOX1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241935E7

N° de cat : 84700-1-RR

Synonymes

MOX1, MOX 1, Mitogenic oxidase 1, GP91 2, EC:1.6.3.-

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Galerie de données de validation

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  WB analysis using 84700-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84700-1-RR (NOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84700-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84700-1-RR against Human NOX1 were performed. The affinity constant is 0.364 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Caco-2, cellules COLO 320, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84700-1-RR cible NOX1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NOX1 Protéine recombinante Ag12187
• Nom complet: NADPH oxidase 1
• Masse moléculaire calculée: 564 aa, 65 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC075014
• Symbole du gène: NOX1
• Identification du gène (NCBI): 27035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NNOX1(NADPH oxidase 1) is also named as MOX1, NOH1 and belongs to the reactive oxygen species (ROS)-generating NADPH oxidase family. It plays a role in host defense, cell growth and differentiation, cell migration, and malignant transformation(PMID:20351171). It also can mediate oncogenic Ras-induced upregulation of VEGF and angiogenesis by activating Sp1 through Ras-ERK-dependent phosphorylation of Sp1(PMID:18454179). It has 3 isoforms produced by alternative splicing with the molecular weight of 65 kDa, 22 kDa and 59 kDa. The full length protein has two glycosylation sites. It always can be detected two bands of 63 kDa and 55 kDa in cells by western blot or another lower band of 45 kDa which is not likely due to alternative translation or splice variation, but as adjustment for the signal peptide and tag removal(PMID:17560373) or unspecifc(PMID:17460729).

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