Anticorps Polyclonal de lapin anti-Nucleoside phosphorylase

Nucleoside phosphorylase Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18009-1-AP

Synonymes

PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837

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Galerie de données de validation

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  WB analysis using 18009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18009-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 18009-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules Raji, cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

18009-1-AP cible Nucleoside phosphorylase dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nucleoside phosphorylase Protéine recombinante Ag12507
• Nom complet: nucleoside phosphorylase
• Masse moléculaire calculée: 289 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC106074
• Symbole du gène: Purine nucleoside phosphorylase
• Identification du gène (NCBI): 4860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Purine nucleoside phosphorylases (PNP) is a ubiquitous enzyme of purine metabolism that functions in the salvage pathway, including even those of protozoan parasites, thus enabling the cells to utilize purine bases recovered from metabolized purine ribo- and deoxyribonucleosides to synthesize purine nucleotides (PMID: 11337031, 23332162). PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. hPNP is widely distributed in various tissues and cells of the human body, with the highest activity in kidney, peripheral lymphocytes and granulocytes (PMID: 38701712).

Publications

Species	Application	Title
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
		J Biol Chem; Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients; Authors - Teresa W -M Fan
canine	WB	Biologicals; CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells; Authors - Zhenbin Liu
rat	WB,IF	Int Immunopharmacol; Spermidine attenuates monocrotaline-induced pulmonary arterial hypertension in rats by inhibiting purine metabolism and polyamine synthesis-associated vascular remodeling; Authors - Yu-Jing Chen
mouse,human	WB	Cell; De novo and salvage purine synthesis pathways across tissues and tumors; Authors - Diem H Tran
mouse,human	WB	J Ethnopharmacol; Diosmetin-7-O-β-D-glucopyranoside from Pogostemonis Herba alleviated SARS-CoV-2-induced pneumonia by reshaping macrophage polarization and limiting viral replication; Authors - Yun-Lu Xu

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