Anticorps Polyclonal de lapin anti-NPC1L1

NPC1L1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28085-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28085-1-AP (same clone as 28085-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28085-1-AP (NPC1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28085-1-PBS cible NPC1L1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPC1L1 Protéine recombinante Ag26277
• Nom complet: NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• Masse moléculaire calculée: 149 kDa
• Poids moléculaire observé: 140-149 kDa
• Numéro d’acquisition GenBank: NM_001101648
• Symbole du gène: NPC1L1
• Identification du gène (NCBI): 29881
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NPC1 like intracellular cholesterol transporter 1 (NPC1L1) is a multi-pass membrane protein which has 1332 amino acids and containing a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD). It is only found in the tubular membranes of primate hepatocytes and the brush-border membranes of the small intestine of mammals. PC1L1 is a key protein for intestinal absorption of cholesterol and plays an important role in cholesterol balance. Cholesterol contributes to cell proliferation, invasion and latency, and is essential for tumor formation and growth, so NPC1L1 plays a critical role in tumor development and spread (PMID: 36034854).

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