Anticorps Polyclonal de lapin anti-NPHP5/IQCB1

NPHP5/IQCB1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15747-1-AP

Synonymes

IQCB1, NPHP5, IQ calmodulin-binding motif-containing protein 1, IQ motif containing B1, IQCB 1

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Galerie de données de validation

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  WB analysis using 15747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15747-1-AP (NPHP5,IQCB1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15747-1-AP (NPHP5/IQCB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15747-1-AP

  IP result of anti-NPHP5/IQCB1 (IP:15747-1-AP, 4ug; Detection:15747-1-AP 1:1000) with HEK-293 cells lysate 1280 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

15747-1-AP cible NPHP5/IQCB1 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPHP5/IQCB1 Protéine recombinante Ag8203
• Nom complet: IQ motif containing B1
• Masse moléculaire calculée: 598 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC005806
• Symbole du gène: IQCB1
• Identification du gène (NCBI): 9657
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.

Publications

Species	Application	Title
human	IF	Hum Mol Genet; Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis.; Authors - Barbelanne Marine M
human		Mol Biol Cell; Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.; Authors - Antonia Wiegering

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