Anticorps Recombinant de lapin anti-NPL

NPL Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

243037E2

N° de cat : 85608-3-PBS

Synonymes

c112, C1orf13, EC:4.1.3.3, N acetylneuraminate lyase, N-acetylneuraminate lyase

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Galerie de données de validation

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  WB analysis using 85608-3-RR (same clone as 85608-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85608-3-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 85608-3-RR (same clone as 85608-3-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85608-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85608-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85608-3-RR against Human NPL were performed. The affinity constant is 4.26 nM.

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Informations sur le produit

85608-3-PBS cible NPL dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NPL Protéine recombinante Ag10137
• Nom complet: N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
• Masse moléculaire calculée: 27 kDa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC058003
• Symbole du gène: NPL
• Identification du gène (NCBI): 80896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.

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