Anticorps Polyclonal de lapin anti-B23/NPM1

• Phare
• Validé par KD/KO

B23/NPM1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (2)

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 10306-1-AP

Synonymes

B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Galerie de données de validation

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  WB analysis using 10306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 10306-1-AP

  WB result of anti-NPM1 (10306-1-AP) in different cell lysates.

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  WB analysis using 10306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10306-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10306-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10306-1-AP

  IP Result of anti-B23 (IP:10306-1-AP, 4ug; Detection:10306-1-AP 1:2000) with Jurkat cells lysate 3200ug.

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 10306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using B23/NPM1 antibody (10306-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of HeLa using 10306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10306-1-AP (B23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, multi-cellules
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 12 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10306-1-AP cible B23/NPM1 dans les applications de WB, IP, IHC, IF, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B23/NPM1 Protéine recombinante Ag0286
• Nom complet: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC002398
• Symbole du gène: NPM1
• Identification du gène (NCBI): 4869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse		EMBO Mol Med; Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.; Authors - Masayuki Ide
human	chIP	Cancer Res; LNMICC promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism.; Authors - Chunliang Shang
human	IF	Int J Mol Sci; TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.; Authors - Ahmed Hassan
human	WB,IF	Mol Ther Nucleic Acids; RPL32 Promotes Lung Cancer Progression by Facilitating p53 Degradation.; Authors - Jiansheng Xie
human	WB	iScience; Elevated high-mannose N-glycans hamper endometrial decidualization; Authors - Siyi Chen ; KD Validated

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