Anticorps Monoclonal anti-B23/NPM1

• Phare
• Validé par KD/KO

B23/NPM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(46)

CloneNo.

4F12A3

Avis (1)

N° de cat : 60096-1-Ig

Synonymes

B23, NPM1, 4F12A3, NPM, Nucleophosmin

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Galerie de données de validation

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  WB analysis using 60096-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 60096-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60096-1-Ig

  WB result of B23/NPM1 antibody (60096-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B23/NPM1 transfected HepG2 cells.

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  WB analysis using 60096-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60096-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60096-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60096-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60096-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60096-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60096-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60096-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 60096-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 5 publications below
• IF: See 23 publications below
• IP: See 4 publications below
• ChIP: See 1 publications below

Informations sur le produit

60096-1-Ig cible B23/NPM1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: B23/NPM1 Protéine recombinante Ag7415
• Nom complet: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC002398
• Symbole du gène: NPM1
• Identification du gène (NCBI): 4869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; lncRNAs maintain the functional phase state of nucleolar prion-like protein to facilitate rRNA processing; Authors - Yu-Meng Sun
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao
		Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
human	WB,IF	Nat Commun; A redox mechanism underlying nucleolar stress sensing by nucleophosmin.; Authors - Kai Yang ; KD Validated
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Aging Cell; Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.; Authors - Patricia Gomez-Suaga

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Was used for nucleolar staining, very strong specific signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

B23/NPM1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:60096-1-Ig)

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