Anticorps Recombinant de lapin anti-NPM3

NPM3 Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242863G12

N° de cat : 85156-5-PBS

Synonymes

242863G12, nucleophosmin/nucleoplasmin, 3, Nucleoplasmin 3, Nucleoplasmin-3, PORMIN

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Galerie de données de validation

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  IF Staining of HeLa using 85156-5-RR (same clone as 85156-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPM3 antibody (85156-5-RR, Clone: 242863G12 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85156-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85156-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85156-5-RR against Human NPM3 were performed. The affinity constant is 1.98 nM.

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Informations sur le produit

85156-5-PBS cible NPM3 dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NPM3 Protéine recombinante Ag2564
• Nom complet: nucleophosmin/nucleoplasmin, 3
• Masse moléculaire calculée: 178 aa, 19 kDa
• Numéro d’acquisition GenBank: BC054868
• Symbole du gène: NPM3
• Identification du gène (NCBI): 10360
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795).

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