Anticorps Polyclonal de lapin anti-NPPA

NPPA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27426-1-PBS

Synonymes

ANP, PND, ANF, ATFB6, Atrial natriuretic factor

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Galerie de données de validation

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  WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 27426-1-AP (same clone as 27426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 27426-1-AP (same clone as 27426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27426-1-PBS cible NPPA dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPPA Protéine recombinante Ag26692
• Nom complet: natriuretic peptide precursor A
• Masse moléculaire calculée: 153 aa, 17 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC005893
• Symbole du gène: NPPA
• Identification du gène (NCBI): 4878
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.

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