Anticorps Polyclonal de lapin anti-NPRL2

• Phare
• Validé par KD/KO

NPRL2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 10157-1-AP

Synonymes

G21 protein, Gene 21 protein, NPRL2, tumor suppressor candidate 4, TUSC4

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Galerie de données de validation

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  WB analysis of HeLa using 10157-1-AP

  WB result of TUSC4 antibody (10157-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC4 transfected HeLa cells.

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  WB analysis of mouse pancreas using 10157-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10157-1-AP (TUSC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse pancreas using 10157-1-AP

  IP result of anti-NPRL2 (IP:10157-1-AP, 3ug; Detection:10157-1-AP 1:500) with mouse pancreas tissue lysate 4000ug.

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  IHC staining of human lung cancer using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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  IHC staining of human skeletal muscle using 10157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10157-1-AP (NPR2L,TUSC4 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu pancréatique de souris, cellules HeLa
• Résultats positifs en IP: tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de muscle squelettique humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10157-1-AP cible NPRL2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPRL2 Protéine recombinante Ag0210
• Nom complet: tumor suppressor candidate 4
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC002791
• Symbole du gène: TUSC4
• Identification du gène (NCBI): 10641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NPRL2 (NPR2 Like, GATOR1 Complex Subunit) is a Protein Coding gene associated with Epilepsy, Familial Focal. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. Homologous to yeast nitrogen permease (candidate tumor suppressor) (NM_006545, BC021984) gene encodes a protein of molecular mass of 50 kDa. Data by using overlapping and nested homozygous deletions methods indicate that this protein might be a tumor suppressor involved in the development of lung cancers.

Publications

Species	Application	Title
human	WB, IHC	Cancer Res; TUSC4 functions as a tumor suppressor by regulating BRCA1 stability.; Authors - Yang Peng
human	WB	Mol Carcinog; Downregulation of nitrogen permease regulator like-2 activates PDK1-AKT1 and contributes to the malignant growth of glioma cells.; Authors - Ning Huang
human	WB	Cancer Sci; TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling.; Authors - Kurata Atsuo A ; KD Validated
human	IHC, WB, IF, CoIP	Exp Cell Res; NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.; Authors - Xin Zhao
human,mouse	WB,IHC	Prostate; PMID: 30178500; Authors - Zhixiong Chen
human,mouse	WB,IF	Exp Cell Res; PMID: 32234375; Authors - Shengjun Luo ; KD Validated

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