Anticorps Polyclonal de lapin anti-NPTX2

• Phare
• Validé par KD/KO

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(20)

Avis (2)

N° de cat : 10889-1-AP

Synonymes

NP-II, NP2, Neuronal pentraxin-2, Neuronal pentraxin II

---

Galerie de données de validation

• 

  WB analysis using 10889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10889-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10889-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10889-1-AP

  IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10889-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 10889-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules PC-3, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10889-1-AP cible NPTX2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPTX2 Protéine recombinante Ag1326
• Nom complet: neuronal pentraxin II
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC009924
• Symbole du gène: NPTX2
• Identification du gène (NCBI): 4885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

Publications

Species	Application	Title
human	WB,IHC,IF	J Invest Dermatol; NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail; Authors - Tong Kang
mouse	IHC,IF	Neuropsychopharmacology; NPTX2 is a key component in the regulation of anxiety.; Authors - Simon Chang ; KO Validated
mouse	IF	Front Immunol; Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice.; Authors - Réka Á Kovács
human	IF	J Neurochem; Quantitative proteomic analysis of the frontal cortex in Alzheimer's disease.; Authors - Gajanan Sathe
human	WB,IHC	J Cancer; Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer.; Authors - Yixi Su
human	WB,IHC	Mol Biol Rep; The neuronal pentraxin II gene (NPTX2) inhibit proliferation and invasion of pancreatic cancer cells in vitro.; Authors - Zhang Ling L

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Xin (Verified Customer) (10-10-2022)

Very good with WB to detect endogenous NPTX2 with a clean and clear band with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

NPTX2 Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:10889-1-AP)

FH

Jerome (Verified Customer) (05-07-2019)

This protein should be in the secretory pathway but instead the antibody stains the nucleus

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 100
• Cell Tissue Type: Hela Cells

NPTX2 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:10889-1-AP)

{{ptg:RelatedPrimaryAntibodies}}