Anticorps Polyclonal de lapin anti-NPTX2

• Phare
• Validé par KD/KO

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10889-1-PBS

Synonymes

Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

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Galerie de données de validation

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  WB analysis using 10889-1-AP (same clone as 10889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10889-1-AP (same clone as 10889-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10889-1-AP (same clone as 10889-1-PBS)

  IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10889-1-PBS cible NPTX2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NPTX2 Protéine recombinante Ag1326
• Nom complet: neuronal pentraxin II
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC009924
• Symbole du gène: NPTX2
• Identification du gène (NCBI): 4885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

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