Anticorps Monoclonal anti-NQO1

NQO1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E5G7

N° de cat : 67240-1-PBS

Synonymes

1E5G7, Azoreductase, DHQU, DIA 4, DIA4

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Galerie de données de validation

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  WB analysis using 67240-1-Ig (same clone as 67240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67240-1-Ig (same clone as 67240-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67240-1-Ig (same clone as 67240-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67240-1-Ig (same clone as 67240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67240-1-Ig (same clone as 67240-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67240-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67240-1-PBS cible NQO1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NQO1 Protéine recombinante Ag28933
• Nom complet: NAD(P)H dehydrogenase, quinone 1
• Masse moléculaire calculée: 274 aa, 31 kDa
• Poids moléculaire observé: 29-31 kDa
• Numéro d’acquisition GenBank: BC007659
• Symbole du gène: NQO1
• Identification du gène (NCBI): 1728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

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