Anticorps Recombinant de lapin anti-NQO1

NQO1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251713C3

N° de cat : 86693-2-RR

Synonymes

Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase

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Galerie de données de validation

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  WB analysis using 86693-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86693-2-RR

  HSC-T6 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86693-2-RR

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86693-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86693-2-RR against Human NQO1 were performed. The affinity constant is 1.80 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HSC-T6, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86693-2-RR cible NQO1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NQO1 Protéine recombinante Ag2009
• Nom complet: NAD(P)H dehydrogenase, quinone 1
• Masse moléculaire calculée: 274 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC007659
• Symbole du gène: NQO1
• Identification du gène (NCBI): 1728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)

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