Anticorps Polyclonal de lapin anti-NQO2

• Phare
• Validé par KD/KO

NQO2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 15767-1-AP

Synonymes

DHQV, DIA6, NQO2, NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, QR2, Quinone reductase 2

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Galerie de données de validation

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  WB analysis using 15767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15767-1-AP

  IP Result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse testis using 15767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15767-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below

Informations sur le produit

15767-1-AP cible NQO2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NQO2 Protéine recombinante Ag8403
• Nom complet: NAD(P)H dehydrogenase, quinone 2
• Masse moléculaire calculée: 231 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC006096
• Symbole du gène: NQO2
• Identification du gène (NCBI): 4835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

Publications

Species	Application	Title
human	WB	Antioxidants (Basel); Apigenin and Luteolin Regulate Autophagy by Targeting NRH-Quinone Oxidoreductase 2 in Liver Cells.; Authors - Elzbieta Janda
human	WB	J Exp Clin Cancer Res; PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug.; Authors - Tiantian He
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
rat	WB	Cell Physiol Biochem; Downregulation of quinone reductase 2 attenuates vascular smooth muscle cells proliferation and neointimal formation in balloon injured rat carotid artery.; Authors - Zhang Xi-wen XW ; KD Validated
human	WB	Life Sci Alliance; Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosis; Authors - Tian-Xiang Wang
human	WB	J Neural Transm (Vienna); The specific NQO2 inhibitor, S29434, only marginally improves the survival of dopamine neurons in MPTP-intoxicated mice; Authors - Maeva Vallucci