Anticorps Polyclonal de lapin anti-NQO2

• Phare
• Validé par KD/KO

NQO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15767-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 15767-1-AP (same clone as 15767-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  IP result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

15767-1-PBS cible NQO2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NQO2 Protéine recombinante Ag8403
• Nom complet: NAD(P)H dehydrogenase, quinone 2
• Masse moléculaire calculée: 231 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC006096
• Symbole du gène: NQO2
• Identification du gène (NCBI): 4835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

{{ptg:RelatedPrimaryAntibodies}}