Anticorps Polyclonal de lapin anti-NR1D1

• Phare
• Validé par KD/KO

NR1D1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14506-1-PBS

Synonymes

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

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Galerie de données de validation

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  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14506-1-PBS cible NR1D1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR1D1 Protéine recombinante Ag5964
• Nom complet: nuclear receptor subfamily 1, group D, member 1
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC056148
• Symbole du gène: NR1D1
• Identification du gène (NCBI): 9572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

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