Anticorps Polyclonal de lapin anti-NR1D2

• Phare
• Validé par KD/KO

NR1D2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13906-1-AP

Synonymes

BD73, EAR 1R, EAR-1R, Rev erb beta, Rev-erb alpha-related receptor

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Galerie de données de validation

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  WB analysis of Jurkat using 13906-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 13906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NR1D2 antibody (13906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

13906-1-AP cible NR1D2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR1D2 Protéine recombinante Ag4881
• Nom complet: nuclear receptor subfamily 1, group D, member 2
• Masse moléculaire calculée: 579 aa, 65 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC045613
• Symbole du gène: NR1D2
• Identification du gène (NCBI): 9975
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Sci Rep; The functional and predictive roles of miR-210 in cryptorchidism.; Authors - Zhengzheng Duan
mouse	WB	Am J Physiol Heart Circ Physiol; Differential effects of REV-ERBα/β agonism on cardiac gene expression, metabolism, and contractile function in a mouse model of circadian disruption.; Authors - Sobuj Mia
human	WB	Xenobiotica; Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.; Authors - Min Chen
human	WB	Cell Death Dis; SR9009 inhibits lethal prostate cancer subtype 1 by regulating the LXRα/FOXM1 pathway independently of REV-ERBs; Authors - Hang Xu ; KD Validated
rat,human	WB	NPJ Biofilms Microbiomes; Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling; Authors - Shang Li
mouse	IHC	J Biomed Sci; Secretome from estrogen-responding human placenta-derived mesenchymal stem cells rescues ovarian function and circadian rhythm in mice with cyclophosphamide-induced primary ovarian insufficiency; Authors - Duy-Cuong Le

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