Anticorps Polyclonal de lapin anti-NR2E3

• Phare
• Validé par KD/KO

NR2E3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14246-1-AP

Synonymes

ESCS, NR2E3, PNR, rd7, RNR, RP37

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Galerie de données de validation

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  WB analysis using 14246-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14246-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14246-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14246-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14246-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 14246-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of photoreceptor progenitor cells using 14246-1-AP

  IF result of NR2E3 antibody (14246-1-AP, 1:50) with human photoreceptor progenitors cells.

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  FC experiment of photoreceptor progenitor cells using 14246-1-AP

  FC result of NR2E3 antibody (14246-1-AP, 1:100) with human photoreceptor progenitors cells. Blue, isotype control; Black=passage 2; Red=passage 4.

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Applications testées

• Résultats positifs en WB: tissu rétinien de souris, cellules HepG2, cellules Y79, tissu rétinien de rat
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules progénitrices photoréceptrices
• Résultats positifs en cytométrie: cellules progénitrices photoréceptrices

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:100
• Flow Cytometry (FC): FC : ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

14246-1-AP cible NR2E3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR2E3 Protéine recombinante Ag5503
• Nom complet: nuclear receptor subfamily 2, group E, member 3
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC041421
• Symbole du gène: NR2E3
• Identification du gène (NCBI): 10002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]

Publications

Species	Application	Title
mouse	IHC	PLoS Genet; Distinct and Atypical Intrinsic and Extrinsic Cell Death Pathways between Photoreceptor Cell Types upon Specific Ablation of Ranbp2 in Cone Photoreceptors.; Authors - Cho Kyoung-In KI
mouse	WB	J Biol Chem; Differential Loss of Prolyl-Isomerase or Chaperone Activity of Ran-binding protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis.; Authors - Kyoung-In Cho
human	WB	Sci Rep; Loss of NR2E3 represses AHR by LSD1 reprogramming, is associated with poor prognosis in liver cancer.; Authors - Tilak Khanal
mouse	WB	FASEB J; NR2E3 is a key component in p53 activation by regulating a long noncoding RNA DINO in acute liver injuries.; Authors - Tilak Khanal ; KO Validated
zebrafish	IHC	Am J Pathol; Dysregulation of Spliceosomes Complex Induces Retinitis Pigmentosa-Like Characteristics in sf3b4-Depleted Zebrafish; Authors - Zulvikar Syambani Ulhaq