Anticorps Monoclonal anti-NR2F6

• Phare
• Validé par KD/KO

NR2F6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H2B8

N° de cat : 60117-2-PBS

Synonymes

2H2B8, EAR 2, EAR2, EAR-2, ERBAL2

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Galerie de données de validation

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  WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

  WB result of NR2F6 antibody (60117-2-Ig, 1:1000) with si-Control and si-NR2F6 transfected HeLa cells. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  WB analysis using 60117-2-Ig (same clone as 60117-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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Informations sur le produit

60117-2-PBS cible NR2F6 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NR2F6 Protéine recombinante Ag7614
• Nom complet: nuclear receptor subfamily 2, group F, member 6
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC002669
• Symbole du gène: NR2F6
• Identification du gène (NCBI): 2063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development (PMID:15875024). NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) (PMID: 12690040). It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity (PMID:18701084).

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