Anticorps Recombinant de lapin anti-NR2F6

NR2F6 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251076E2

N° de cat : 86371-1-PBS

Synonymes

EAR 2, EAR2, EAR-2, ERBAL2, V erbA related protein 2

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Galerie de données de validation

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  WB analysis using 86371-1-RR (same clone as 86371-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86371-1-RR (NR2F6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86371-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 86371-1-RR (same clone as 86371-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NR2F6 antibody (86371-1-RR, Clone: 251076E2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86371-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86371-1-RR (same clone as 86371-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NR2F6 antibody (86371-1-RR, Clone: 251076E2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86371-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86371-1-PBS cible NR2F6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NR2F6 Protéine recombinante Ag7614
• Nom complet: nuclear receptor subfamily 2, group F, member 6
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC002669
• Symbole du gène: NR2F6
• Identification du gène (NCBI): 2063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development (PMID:15875024). NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) (PMID: 12690040). It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity (PMID:18701084).

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