Anticorps Polyclonal de lapin anti-NR4A1

• Phare
• Validé par KD/KO

NR4A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 25851-1-AP

Synonymes

Early response protein NAK1, GFRP1, HMR, N10, NAK 1, NAK1, NGFIB, NR4A1, NUR77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST 59, Testicular receptor 3, TR3

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Galerie de données de validation

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  WB analysis of PC-3 using 25851-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25851-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25851-1-AP (NR4A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25851-1-AP

  IP result of anti-NR4A1(IP:25851-1-AP, 4ug; Detection:25851-1-AP 1:500) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse colon using 25851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25851-1-AP (NR4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25851-1-AP (NR4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HepG2, cellules PC-3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de côlon de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

25851-1-AP cible NR4A1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NR4A1 Protéine recombinante Ag23088
• Nom complet: nuclear receptor subfamily 4, group A, member 1
• Masse moléculaire calculée: 598 aa, 64 kDa
• Poids moléculaire observé: 64-68 kDa
• Numéro d’acquisition GenBank: BC016147
• Symbole du gène: NR4A1
• Identification du gène (NCBI): 3164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	FASEB J; The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth.; Authors - Saki Hatano
mouse	WB,IHC	J Cell Mol Med; NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression.; Authors - Dan-Dan Qin ; KO Validated
rat	IF	Phytomedicine; Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis.; Authors - Md Nazmul Haque
human	WB	Front Mol Biosci; Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.; Authors - Wenzhou Liu ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.; Authors - Xiaoyan Wan
rat	WB	Front Physiol; Sleeve Gastrectomy-Induced AMPK Activation Attenuates Diabetic Cardiomyopathy by Maintaining Mitochondrial Homeostasis via NR4A1 Suppression in Rats.; Authors - Songhan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angela (Verified Customer) (06-23-2020)

35ug total protein/lane1- Untransfected HEK2932- Transfection with human NR4A1 expression vector3- Transfection with rat NR4A1 expression vector

• Applications: Western Blot
• Primary Antibody Dilution: 500x
• Cell Tissue Type: HEK293

NR4A1 Antibody Western Blot validation (500x dilution) in HEK293 (Cat no:25851-1-AP)