Anticorps Polyclonal de lapin anti-NR4A3

NR4A3 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55405-1-AP

Synonymes

TEC, NOR1, MINOR, CSMF, CHN

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Galerie de données de validation

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  WB analysis of mouse spleen using 55405-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 55405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55405-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55405-1-AP (NR4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of ROS1728 using 55405-1-AP

  Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using NR4A3 antibody (55405-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, tissu splénique de souris
• Résultats positifs en IF/ICC: cellules ROS1728,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

55405-1-AP cible NR4A3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear receptor subfamily 4, group A, member 3
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: NM_006981
• Symbole du gène: NR4A3
• Identification du gène (NCBI): 8013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Multimodal discovery of bavachinin A: A natural FLT3 agonist for treating thrombocytopenia; Authors - Long Wang
rat	WB	Biology (Basel); CEBPA Overexpression Enhances β-Cell Proliferation and Survival; Authors - Peter N Ellsworth
human	WB,IHC	Aging (Albany NY); Oxidative stress-induced EGR1 upregulation promotes NR4A3-mediated nucleus pulposus cells apoptosis in intervertebral disc degeneration; Authors - Si-Kuan Zheng

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