Anticorps Polyclonal de lapin anti-NRAP

NRAP Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25977-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25977-1-PBS cible NRAP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRAP Protéine recombinante Ag23254
• Nom complet: nebulin-related anchoring protein
• Poids moléculaire observé: 190-200 kDa
• Numéro d’acquisition GenBank: BC126407
• Symbole du gène: NRAP
• Identification du gène (NCBI): 4892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NRAP (Nebulin-related-anchoring protein) is a striated muscle-specific protein that is concentrated at intercalated disks in cardiac muscle and at myotendinous junctions in skeletal muscle. NRAP is a 197 kDa multidomain scaffolding protein from nebulin family with many interation partners such as α-actinin, actin, vinculin and filamin (PMID:21276443). During cardiomyocyte development in the foetal heart, NRAP is involved in myofibrillar assembly and links terminal actin filaments to membrane complexes in the adult heart. Several studies observed that NRAP mutation would contributed to the dilated cardiomyopathy which is a highly heterogeneous disease with almost 100 candidates genes to date (PMID:28611399).

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