Anticorps Recombinant de lapin anti-NRAS

NRAS Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242810G11

N° de cat : 83815-2-PBS

Synonymes

HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1

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Galerie de données de validation

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  WB analysis using 83815-2-RR (same clone as 83815-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83815-2-RR (NRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83815-2-RR (same clone as 83815-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83815-2-RR (same clone as 83815-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83815-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83815-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83815-2-RR against Human NRAS were performed. The affinity constant is 0.152 nM.

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Informations sur le produit

83815-2-PBS cible NRAS dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NRAS Protéine recombinante Ag1081
• Nom complet: neuroblastoma RAS viral (v-ras) oncogene homolog
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC005219
• Symbole du gène: NRAS
• Identification du gène (NCBI): 4893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). NRAS is one member of RAS gene family of oncoproteins, which is commonly mutated in melanoma and hematopoietic cancers via mapped on chromosome 1 (PMID: 2327491, PMID: 26990546). NRAS mediates activation of both mitogen-activated protein kinase (MAPK) and PI3K/AKT/MYC signaling (PMID: 17297468). NRAS induced classical MAPK signaling leads to cyclin D1 expression and cell cycle dysregulation and promotion of prosurvival pathways (PMID:7970723,PMID: 18246127). In addition, NRAS effectively prevents Glycogen Synthase Kinase3 (GSK3)-mediated phosphorylation of MYC via PI3K/AKT, which results in enhanced activity of endogenous MYC protein (PMID: 17297468). Mutational NRAS causes Ras-GTP to be in a state of continuous activation, which results in malignant proliferation and metastasis (PMID: 24985059).

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