Anticorps Recombinant de lapin anti-NRAS

NRAS Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242810G11

N° de cat : 83815-2-RR

Synonymes

HRAS1, EC:3.6.5.2, GTPase NRas, N ras, NRAS1

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Galerie de données de validation

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  WB analysis using 83815-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83815-2-RR (NRAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 83815-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83815-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83815-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83815-2-RR (NRAS antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83815-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83815-2-RR against Human NRAS were performed. The affinity constant is 0.152 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules C6, cellules HEK-293, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire de souris, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83815-2-RR cible NRAS dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NRAS Protéine recombinante Ag1081
• Nom complet: neuroblastoma RAS viral (v-ras) oncogene homolog
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC005219
• Symbole du gène: NRAS
• Identification du gène (NCBI): 4893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). NRAS is one member of RAS gene family of oncoproteins, which is commonly mutated in melanoma and hematopoietic cancers via mapped on chromosome 1 (PMID: 2327491, PMID: 26990546). NRAS mediates activation of both mitogen-activated protein kinase (MAPK) and PI3K/AKT/MYC signaling (PMID: 17297468). NRAS induced classical MAPK signaling leads to cyclin D1 expression and cell cycle dysregulation and promotion of prosurvival pathways (PMID:7970723,PMID: 18246127). In addition, NRAS effectively prevents Glycogen Synthase Kinase3 (GSK3)-mediated phosphorylation of MYC via PI3K/AKT, which results in enhanced activity of endogenous MYC protein (PMID: 17297468). Mutational NRAS causes Ras-GTP to be in a state of continuous activation, which results in malignant proliferation and metastasis (PMID: 24985059).

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