Anticorps Recombinant de lapin anti-NRCAM

NRCAM Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241862G1

N° de cat : 84690-1-PBS

Synonymes

241862G1, hBravo, KIAA0343, neuronal cell adhesion molecule, Neuronal surface protein Bravo

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 84690-1-RR (same clone as 84690-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

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  WB analysis using 84690-1-RR (same clone as 84690-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84690-1-RR (NRCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84690-1-RR (same clone as 84690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84690-1-RR (NRCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84690-1-RR (same clone as 84690-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84690-1-RR (NRCAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84690-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84690-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84690-1-RR against Human NRCAM were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84690-1-PBS cible NRCAM dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NRCAM Protéine recombinante Ag16237
• Nom complet: neuronal cell adhesion molecule
• Masse moléculaire calculée: 1304 aa, 144 kDa
• Poids moléculaire observé: 144 kDa
• Numéro d’acquisition GenBank: BC098401
• Symbole du gène: NRCAM
• Identification du gène (NCBI): 4897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).

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