Anticorps Monoclonal anti-NRF1/nuclear respiratory factor 1

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C2B8

N° de cat : 66832-1-PBS

Synonymes

NRF1, nuclear respiratory factor 1, NRF 1, Alpha-pal, Alpha palindromic-binding protein

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Galerie de données de validation

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  WB analysis using 66832-1-Ig (same clone as 66832-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66832-1-Ig (NRF1/nuclear respiratory factor 1) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66832-1-Ig (same clone as 66832-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66832-1-Ig (NRF1/nuclear respiratory factor 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRF1/nuclear respiratory factor 1 antibody (66832-1-Ig, Clone: 3C2B8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66832-1-Ig (same clone as 66832-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human NRF1/nuclear respiratory factor 1 (66832-1-Ig, Clone:3C2B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66832-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66832-1-PBS cible NRF1/nuclear respiratory factor 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NRF1/nuclear respiratory factor 1 Protéine recombinante Ag28149
• Nom complet: nuclear respiratory factor 1
• Masse moléculaire calculée: 522 aa, 56 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC016925
• Symbole du gène: NRF1
• Identification du gène (NCBI): 4899
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

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