Anticorps Polyclonal de lapin anti-NRG1, isoform SMDF

NRG1, isoform SMDF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 10527-1-AP

Synonymes

NRG1, NRG1A, ARIA, GGF, GGF2

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Galerie de données de validation

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  WB analysis using 10527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10527-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 1x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10527-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10527-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10527-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac de souris, tissu de cervelet de souris, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Informations sur le produit

10527-1-AP cible NRG1, isoform SMDF dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NRG1, isoform SMDF Protéine recombinante Ag0803
• Nom complet: neuregulin 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007675
• Symbole du gène: NRG1
• Identification du gène (NCBI): 3084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The immunogen of this antibody is against NRG1 isoform SMDF.

Publications

Species	Application	Title
rat	WB	ACS Biomater Sci Eng; Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves; Authors - Lei Zhang
mouse	WB,IHC,IF	Ecotoxicol Environ Saf; Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.; Authors - Dongmin Yu
human	IHC	J Exp Clin Cancer Res; miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.; Authors - Dong-Min Shi
mouse	WB	Am J Physiol Renal Physiol; ErbB4 deletion accelerates renal fibrosis following renal injury.; Authors - Fenghua Zeng
Human	WB,IHC	Int J Oncol; NRG1 regulates redox homeostasis via NRF2 in papillary thyroid cancer.; Authors - Ting-Ting Zhang
human	IF	Folia Neuropathol; miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1.; Authors - Chengyan Sun

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