Anticorps Monoclonal anti-NRG1, isoform SMDF

NRG1, isoform SMDF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C6D12

N° de cat : 66492-1-PBS

Synonymes

NRG1, NRG1A, 2C6D12, ARIA, GGF

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Galerie de données de validation

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  WB analysis using 66492-1-Ig (same clone as 66492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 66492-1-Ig (same clone as 66492-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66492-1-Ig (same clone as 66492-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66492-1-Ig (same clone as 66492-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66492-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66492-1-PBS cible NRG1, isoform SMDF dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NRG1, isoform SMDF Protéine recombinante Ag0803
• Nom complet: neuregulin 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007675
• Symbole du gène: NRG1
• Identification du gène (NCBI): 3084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.

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