Anticorps Recombinant de lapin anti-NRG1, isoform SMDF

NRG1, isoform SMDF Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240184G2

N° de cat : 83323-6-PBS

Synonymes

NRG1, NRG1A, 240184G2, ARIA, GGF

---

Galerie de données de validation

• 

  IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 83323-6-RR (same clone as 83323-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 83323-6-RR (NRG1, isoform SMDF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 83323-6-RR (same clone as 83323-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform SMDF antibody (83323-6-RR, Clone: 240184G2 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83323-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83323-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83323-6-RR against Human NRG1, isoform SMDF were performed. The affinity constant is 1.64 nM.

---

Informations sur le produit

83323-6-PBS cible NRG1, isoform SMDF dans les applications de IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NRG1, isoform SMDF Protéine recombinante Ag0803
• Nom complet: neuregulin 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007675
• Symbole du gène: NRG1
• Identification du gène (NCBI): 3084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform SMDF.

{{ptg:RelatedPrimaryAntibodies}}