Anticorps Recombinant de lapin anti-Neurogranin

Neurogranin Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240526B9

N° de cat : 83593-5-PBS

Synonymes

NRGN, 240526B9, hng, NEUG(55-78), Ng

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Galerie de données de validation

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  WB analysis using 83593-5-RR (same clone as 83593-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83593-5-RR (Neurogranin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83593-5-RR (Neurogranin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83593-5-RR (same clone as 83593-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Neurogranin antibody (83593-5-RR, Clone: 240526B9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83593-5-RR (same clone as 83593-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Neurogranin Recombinant antibody (83593-5-RR, Clone:240526B9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83593-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83593-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83593-5-RR against Human NRGN were performed. The affinity constant is 0.304 nM.

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Informations sur le produit

83593-5-PBS cible Neurogranin dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Neurogranin Protéine recombinante Ag0676
• Nom complet: neurogranin (protein kinase C substrate, RC3)
• Masse moléculaire calculée: 7.6 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC002835
• Symbole du gène: Neurogranin
• Identification du gène (NCBI): 4900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neurogranin (formerly designated p17, also known as Ng, RC3 and BICKS) belongs to the neurogranin family. It is a neuron-specific substrate for protein kinase C (PKC). It is a postsynaptic protein that is highly enriched in brain, with restricted expression in the cortex, striatum, hippocampus, thalamus, hypothalamus and olfactory bulb nuclei. Neurogranin binds calmodulin at low levels of calcium, thereby regulating calmodulin-dependent nitric oxide synthase. Neurogranin acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. It binds to calmodulin in the absence of calcium.

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