Anticorps Monoclonal anti-Neuropilin 1

Neuropilin 1 Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc et plus (2)

Applications

WB, IP, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

17

CloneNo.

2H3F6

N° de cat : 60067-1-Ig

Synonymes

BDCA4, CD304, neuropilin 1, NRP, NRP1, VEGF165R

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Galerie de données de validation

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  WB analysis using 60067-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60067-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit heart using 60067-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-231 using 60067-1-Ig

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60067-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 60067-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60067-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 60067-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 60067-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60067-1-Ig (Neuropilin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of human embronic stem cells using 60067-1-Ig

  Confocal immunofluorescent analysis of human embronic stem cells with 60067-1-Ig at dilution of 1:100. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml. This photo is the merge of PE and DAPI. (40X).

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  IF Staining of SH-SY5Y using 60067-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 60067-1-Ig(Neuropilin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of SH-SY5Y using 60067-1-Ig

  1X10^6 SH-SY5Y cells were stained with 0.20 ug/test Anti-Human Neuropilin 1 (60067-1-Ig, Clone:2H3F6) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules A549, cellules HeLa, cellules MDA-MB-231, plasma humain, tissu cardiaque de lapin, tissu cardiaque de porc, tissu cérébral de porc, tissu cérébral humain fœtal
• Résultats positifs en IF: cellules SH-SY5Y, cellules souches embryonnaires humaines
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60067-1-Ig cible Neuropilin 1 dans les applications de WB, IP, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Neuropilin 1 Protéine recombinante Ag0931
• Nom complet: neuropilin 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC007737
• Symbole du gène: NRP1
• Identification du gène (NCBI): 8829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.

Publications

Species	Application	Title
Xenopus	IF	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	IF	Acta Biomater; Semaphorin-3A, Neuropilin-1 and Plexin-A1 in Prosthetic-Particle Induced Bone Loss.; Authors - S Saad
human		J Pathol; Perivascular Neuropilin-1 expression is an independent marker of improved survival in renal cell carcinoma.; Authors - Eric Morin
human	IF	Am J Cancer Res; CMTM6 promotes cell proliferation and invasion in oral squamous cell carcinoma by interacting with NRP1.; Authors - Yang Zheng
mouse	WB	Pharm Res; Dual Receptor Targeting Cell Penetrating Peptide Modified Liposome for Glioma and Breast Cancer Postoperative Recurrence Therapy.; Authors - Yue Qiu
mouse	IF	Exp Eye Res; Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury.; Authors - Victor H Guaiquil

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