Anticorps Monoclonal anti-NSE

• Phare
• Validé par KD/KO

NSE Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6F8G3

N° de cat : 66150-1-PBS

Synonymes

ENO2, 2-phospho-D-glycerate hydro-lyase, 6F8G3, EC:4.2.1.11, Enolase 2

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Galerie de données de validation

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  WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  WB result of NSE antibody (66150-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66150-1-Ig (same clone as 66150-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66150-1-Ig (same clone as 66150-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66150-1-Ig (same clone as 66150-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66150-1-Ig (same clone as 66150-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 66150-1-Ig (same clone as 66150-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66150-1-Ig (NSE antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66150-1-Ig (NSE antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66150-1-Ig (same clone as 66150-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSE antibody (66150-1-Ig, Clone: 6F8G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 66150-1-Ig (same clone as 66150-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NSE (66150-1-Ig, Clone:6F8G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66150-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66150-1-PBS cible NSE dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSE Protéine recombinante Ag19106
• Nom complet: enolase 2 (gamma, neuronal)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC002745
• Symbole du gène: NSE
• Identification du gène (NCBI): 2026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

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