Anticorps Recombinant de lapin anti-NSE

NSE Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250903A11

N° de cat : 86332-1-RR

Synonymes

ENO2, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2, Gamma enolase

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Galerie de données de validation

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  WB analysis using 86332-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86332-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86332-1-RR (ENO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86332-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86332-1-RR against Human NSE were performed. The affinity constant is 1.16 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HT-29, cellules SW480, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86332-1-RR cible NSE dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NSE Protéine recombinante Ag0196
• Nom complet: enolase 2 (gamma, neuronal)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC002745
• Symbole du gène: NSE
• Identification du gène (NCBI): 2026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.

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